Why peg in yeast transformation

Robin A. Woods 1. Access enabled via: An Institution. PDF Full text Related articles. A high-efficiency method for the … more. Citations Recent citations: Sami Havukainen et al. Related articles Based on techniques. See more. Sheerin , , Springer Protocols.

Associated articles R. Daniel Gietz, R. Daniel Gietz, This version , References Ito, H. Schiestl, R. Gietz, R. Yeast transformation. Steel, L. Google Scholar. Guthrie, C. Protocols doi Rose, M. Isolation of genes by complementation in yeast. Methods Enzymol.

Download references. You can also search for this author in PubMed Google Scholar. Correspondence to Robert H Schiestl. Reprints and Permissions. Nat Protoc 2, 35—37 Download citation. Published : 31 January Issue Date : January Anyone you share the following link with will be able to read this content:. Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Applied Biochemistry and Biotechnology Biotechnology for Biofuels Scientific Reports Using yeast in log phase is only important if you need really high transformation efficiency 2 hybrid or library screening.

For routine transformations we dilute yeast and grow hours before transforming not quite log phase. When doing transformations, we incubate the transformation mix for 15 minutes with gentle mixing at 30 degrees, before a 15 minute heat shock.

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